Teaching Basic Immunology Using Flow Cytometry
نویسندگان
چکیده
منابع مشابه
Flow cytometry: basic principles and applications.
Flow cytometry is a sophisticated instrument measuring multiple physical characteristics of a single cell such as size and granularity simultaneously as the cell flows in suspension through a measuring device. Its working depends on the light scattering features of the cells under investigation, which may be derived from dyes or monoclonal antibodies targeting either extracellular molecules loc...
متن کاملDanish Society of Immunology Danish Society for Flow Cytometry
2 Mannan-binding lectin recognizes structures on ischemic reperfused mouse kidneys and is implicated in tissue injury Mette Møller-Kristensen, Weidong Wang, Marieta Ruseva, Steffen Thiel, Søren Nielsen, Kazue Takahashi, Lei Shi, Alan Ezekowitz, Jens Chr. Jensenius, Mihaela Gadjeva. Department of Medical Microbiology and Immunology, Fax: +45 86196128, Tel: +45 89421776, The Water and Salt Resear...
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A flow cytometric method for the quantitation of reticulocytes was refined for routine laboratory use. Blood (2 microliters) is added to 2 ml of 0.4 microM thiazole orange in phosphate buffered saline, incubated at room temperature for 90 minutes, and analysed on a Coulter EPICS Profile flow cytometer, with gating for red cells on the basis of forward and right angled light scatter. Blood (2 mi...
متن کاملA teaching database for diagnosis of hematologic neoplasms using immunophenotyping by flow cytometry.
CONTEXT In the diagnosis of lymphomas and leukemias, flow cytometry has been considered an essential addition to morphology and immunohistochemistry. The interpretation of immunophenotyping results by flow cytometry involves pattern recognition of different hematologic neoplasms that may have similar immunologic marker profiles. An important factor that creates difficulty in the interpretation ...
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T cells are an essential cellular component of the immune system. When T cells encounter antigen and receive co-stimulatory signals, they become activated, proliferate and produce cytokines. Flow cytometry is a valuable research tool in analyzing T cell functions including proliferation, survival and cytotoxicity as well as cytokine production and cell signaling. This article has reviewed the u...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2008
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.22.1_supplement.574.5